The humble clarifier, long seen as little more than a settling tank at the end of a wastewater treatment plant’s coagulation–flocculation stage, may soon get a second act—one with implications that ripple far beyond the treatment process and into the energy sector’s own sustainability goals.
Research led by Andrea Di Cesare of Italy’s National Research Council (CNR) – Water Research Institute (IRSA) suggests that tertiary clarifiers could play a pivotal role in controlling not just bacterial populations, but also the spread of antibiotic resistance genes (ARGs) in treated wastewater. In a study published in *npj Clean Water* (the English translation of *npj Clean Water*), Di Cesare and his team analyzed microbial dynamics in three different wastewater treatment plants using advanced genomic and flow cytometry tools. Their findings challenge the conventional view of clarifiers as passive sedimentation units and point to a more active ecological role.
“Tertiary clarifiers aren’t just removing solids—they’re shaping the entire microbial community,” says Di Cesare. “We found that while fecal bacteria decline, environmental bacteria persist or even increase, and ARGs remain a concern due to potential horizontal gene transfer.”
What makes this particularly significant is the potential to reduce reliance on energy-intensive disinfection processes. Final disinfection—often through chlorination or UV—is a major operational cost and environmental burden in wastewater treatment. By optimizing clarifier retention times and ecological conditions, plants could achieve better microbial control upstream, lowering the intensity (and energy use) of downstream disinfection.
The implications for the energy sector are clear. Wastewater treatment is one of the most energy-intensive processes in municipal infrastructure. Reducing chemical or UV disinfection loads could translate into measurable energy savings—potentially cutting electricity demand for treatment plants that serve millions. It also aligns with broader decarbonization efforts, especially in regions where wastewater utilities are exploring renewable energy integration.
Di Cesare’s team observed that clarifiers function almost like ecological filters, with biofilms acting as reservoirs that stabilize microbial diversity despite fluctuations in influent quality. This resilience suggests that with the right design tweaks—such as controlled retention times or surface modifications—clarifiers could be re-engineered to enhance their microbial filtering capacity.
For plant operators and energy planners alike, the message is timely: a deeper understanding of tertiary clarifiers could unlock dual benefits—improved effluent quality and reduced energy footprints. As wastewater treatment plants increasingly become energy producers (via biogas or thermal energy recovery), this kind of optimization could help balance treatment efficacy with sustainability.
The study, published in *npj Clean Water*, doesn’t claim to replace disinfection entirely, but it does reframe the clarifier as a strategic asset—one that could help the water sector meet stricter microbial and genetic pollution standards without overburdening energy systems.

